UCLA CFAR CORES

Flow Cytometry / Cell Sorting Core

Director:
Beth Jamieson, Ph.D.
310.206.8217
bjamieson@mednet.ucla.edu

The UCLA Flow Cytometry Facility provides instrumentation as well as technical and professional assistance for performing laser-based flow cytometric analysis and sorting. The facility operates seven BD Biosciences instruments: one three-color FACScan, one four-color FACSCalibur, one modified five-color FACScan and one UV-capable, three-laser BD LSR analytic flow cytometer, two three-laser FACSDiVa high-speed sorters, and one nine-color BD FACSAria bench-top high-speed sorter. All cell sorters have been tested for aerosol containment and are available for sorting of unfixed and potentially biohazardous cells, e.g., from HIV-infected persons. One extra G4 Macintosh computer is available for investigators to do their own data analysis in the core laboratory. The facility also operates an AutoMACS magnetic cell separation device manufactured by Miltenyi Biotec which provides rapid large-volume cell separations for investigators. The AutoMACS separator is contained in a Class II Biosafety cabinet and is particularly suited for isolation of cell subpopulations from known infectious samples. Classes in basic flow cytometry and nucleic acid analysis are offered by core staff. Details are available on the core website at http://cyto.mednet.ucla.edu.