UCLA CFAR CORES

Flow Cytometry / Cell Sorting Core

Director:
Beth Jamieson, Ph.D.
310.206.8217
jamieson@mednet.ucla.edu

The UCLA Flow Cytometry Facility provides instrumentation and technical and professional assistance for performing laser-based flow cytometric analysis and sorting.  The facility operates five analyzers: one three-color FACScan, one four-color FACSCalibur, one modified five-color FACScan, and two BD LSRII cytometers, one with 12-color and one 14-color capability for rapid state-of-the-art polychromatic flow cytometry.  The facility also has three high-speed cell sorters, one nine-color FACSAria II, one ten-color FACSAria II, and one three-laser FACSDiVa instrument.  All sorters have been tested for aerosol containment and are available for sorting of unfixed and potentially biohazardous cells, e.g., from HIV-infected persons.  One extra G4 Macintosh computer is available for investigators to perform their own data analysis in the core laboratory.  The facility also operates a RoboSep automated magnetic cell separator which provides sample preparation and large volume cell separations for investigators.  RoboSep is contained in a Class II Biosafety cabinet and is particularly suited for isolation of cell subpopulations from known infectious samples.  Classes in basic flow cytometry and nucleic acid analysis are offered by core staff. Details are available on the core web site at http://cyto.mednet.ucla.edu.